Journal: NPJ Breast Cancer
Article Title: Targeting GD2 with naxitamab overcomes GD3 synthase-driven immune suppression in triple-negative breast cancer
doi: 10.1038/s41523-025-00843-7
Figure Lengend Snippet: A Analysis of GD3S ( ST8SIA1 ) mRNA expression in METABRIC and TCGA (pan cancer) BC patient datasets shows significant correlation with immune checkpoint inhibitors. In the METABRIC dataset, Pearson’s correlation coefficients indicate significant correlations of GD3S mRNA expression with CTLA-4 ( r = 0.4; P < 0.001), LAG3 ( r = 0.33; P < 0.001), PD1 ( r = 0.3; P < 0.001), CD25 ( r = 0.38; P < 0.001), and SIRPα ( r = 0.36; P < 0.001). Similarly, in the TCGA dataset, GD3S mRNA expression was significantly correlated with CTLA-4 ( r = 0.47; P < 0.001), LAG3 ( r = 0.33; P < 0.001), PD1 ( r = 0.38; P < 0.001), CD25 ( r = 0.45; P < 0.001), and SIRPα ( r = 0.56; P < 0.001). B Representative IHC images obtained by sequential multiplex immunofluorescence images (Lunaphore COMET) from FFPE samples of TNBC patients with high or low GD3S expression ( n = 20 total) were used to compare immune infiltration patterns, showing distinct fluorescence intensities for PANK, Ki67, CD163, CD86, CD56, CD8, CD4, FOXP3, and GD3S within tumor tissues. C H-scores for GD3S expression in BC patients categorized into high and low GD3S expression groups ( ****P < 0.0001). Immune cell counts using Visiopharm IHC image analysis software show differences in the expression of CD8, CD4, CD86, and CD56 between the high and low GD3S expression groups ( ****P < 0.0001; **P < 0.01). Scale bar in IHC images represents 55 µm with 20x magnification. Bar graphs display means ± standard deviations.
Article Snippet: We also purchased the following antibodies: pan-Cytokeratin (Proteintech, cat. #26411-1-AP, rabbit host) along with GD3S (Proteintech, cat. #24918-1-AP, rabbit host), CD45 (Fortislife, clone BL-178-1267, cat. #A700-012, rabbit host), Ki67 (Bethyl, clone BLR021E, cat. #A700-021CF, rabbit host), CD3 (Fortislife, clone BL-298-5012, cat. #A700-016F, rabbit host), CD8 (Fortislife, clone c8/144B, cat. #A500-012A, mouse host), CD4 (Bethyl, clone BL-155-1C11, cat. #A700-015, rabbit host), FOXP3 (Bethyl, clone BLR034F, cat. #A700-034, rabbit host), CD68 (Fortislife, cat. #A500-018ACF, mouse host), CD163 (Invitrogen, clone I0D6, cat. #MA5-11458, mouse host), CD86 (CST, clone E2G8P, cat. #76755SF, rabbit host), CD56 (Fortislife, clone BLR152J, cat. #A700-152, rabbit host) and human IgG1 Kappa antibody (hIgG, cat. #0151K-14, SouthernBiotech).
Techniques: Expressing, Multiplex Assay, Immunofluorescence, Fluorescence, Software
